Snake Fungal Disease

Disease Facts

Causitive agent: Ophidiomyces ophiodiicola Fungus
Species affected: Pit vipers (Eastern Massasauga, Timber Rattlesnake and Cotton Mouth), nonvenomous colubrid snakes (Black rat snakes, Milk Snake, Garter snakes)
Zoonotic risk: No
Transmission: environment-to-snake or snake to snake
Seaonality: lesions have been seen on snakes emerging from hibernation, cases also identified in spring and summer
Mortality rate: estimated ~ 40%
Clinical Signs: skin lesions along head, neck and body, pneumonia, ocular infections
Diagnosis: identification of fungus in a skin/scale biopsy along with positive culture or DNA test (PCR)
Treatment: Antifungal medications and supportive care

Causitive Agent and Affected Species

The organism responsible is the fungus Ophidiomyces ophiodiicola. It is characterized as an environmental saprobe, meaning that it normally feeds on decaying organic matter in the environment. This is evident due to its highly tolerant nature; it can thrive in a wide pH range (5 – 11), it is drought tolerant, and can utilize a number of complex carbon, nitrogen and sulfur compounds. Evidence that this organism is a saprobe makes it likely that infection of snakes is of opportunistic nature.

O. ophiodiicola is within the family Onygenacea and is closely related to Chrysosporium anamorph Nannizziopsis vriessii (CANV) complex; many early reports of snake fungal disease identify CANV as the causative organism but the fungus has since been reclassified. Snake Fungal Disease was first definitively identified in a population of Timber Rattlesnakes residing in New Hampshire in 2006. Since then, both colubrids and pit vipers in Eastern and Midwestern United States have been identified with SFD. However, recent advances in molecular diagnostics have allowed identification of cases dating back as far as 1986.

Affected species include milk snakes, black rat snakes, garter snakes, timber rattle snake, eastern massasauga, cotton mouth snakes and black racer snakes.


The fungus is believed to be spread from the environment-to-snake. Since O. ophiodiicola is an environmental saprobe, it is likely that the fungus resides in the soil. Spread of the fungus may occur through anthropogenic causes such as people moving contaminated soil imbedded in clothing or shoes. There have been a number of cases of captive snake populations becoming infected as well. This brings to question the origin of the fungus and that it may have come to the United States via the exotic captive snake trade. There is no definitive evidence of snake to snake transmission. However, since it is a lesion that predominates on the skin, it is possible that shedding of fungal particles from the external lesions can result in further dissemination of the disease, especially in species that share dens.

Several studies have indicated that temperature is a significant factor affecting the growth of O. ophiodiicola. This suggests that populations hibernating in the lower thermal range of 0 °C - 10 °C should have a reduced infection during the spring and summer than snakes that hibernate in the upper thermal range of 0 °C - 10 °C. In addition, data suggest that with increasing global temperatures, snake populations will be more vulnerable to O.ophiodiicola.

Clinical Signs

Incubation period is between 30 to 37 days with some showing clinical signs as early as day 12 of inoculation. The characteristic clinical sign of SFD is facial swelling. The disease progresses from the nasal cavity internally via the eyes, throat and lungs causing ocular infections and pneumonia in some cases. The fungus additionally spreads externally along the neck, body and tail forming scattered nodules under the scales. In rare cases where there are wounds secondary to the infection, the fungus can penetrate the body and cause a systemic fungal infection resulting in nodules on the coelomic fat pad, kidneys, liver and air sac. Experimental data shows snakes surviving an average of 90 days with SFD and having a 40% mortality rate.


Diagnosis of SFD involves visual identification of the fungus or lesions consistent with an infection as well as laboratory identification of the fungus. Methods to identify the fungus include histopathological examination via skin biopsy, fungal culture and real-time or quantitative polymerase chain reaction (rtPCR and qPCR).


Due to its close relation to Chrysosporium anamorph Nannizziopsis vriessii (CANV) complex, O. ophiodiicola infections may have been misdiagnosed previously due to a lack of specific testing for the fungus. Treatment with antifungal medications has not been successful in colubrid snakes.


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